Table 1.

S. cerevisiae strains used in this studya

StrainGenotypeSource or reference
W303-1A MAT a 67
W303-1B MATα 67
YKH10a MAT a ADE2 K. Huang
W1588-4C MAT a RAD5 R. Rothstein
W1588-4A MATα RAD5 R. Rothstein
YHK595-1C MATα rad51::LEU2 RAD5 H. Klein
YHK598-8B MATα rad57::LEU2 RAD5medH. Klein
LSY563 MAT a rad59::LEU2 Y. Bai
B404-4D MATα rad51::HIS3 rad59::LEU2 lys2 Y. Bai
Y301 MAT a rad53-21 S. Elledge
LSY693 MAT a ADE2 met17-s This study
LSY694 MAT a met17::ADE2 This study
LSY695-2B MATα ADE2 met17-s RAD5 This study
LSY695-7D MAT a ADE2 met17-s RAD5 This study
LSY696-1C MATα met17::ADE2 RAD5 This study
LSY696-7A MAT a met17::ADE2 RAD5 This study
LSY715 MATα rad52::LEU2 This study
LSY826 MAT a rad51::LEU2 met17-s ADE2 RAD5 This study
LSY827 MAT a rad51::LEU2 met17::ADE2 RAD5 This study
LSY718 MAT a rad52::LEU2 met17-s ADE2 RAD5 This study
LSY723 MAT a rad52::LEU2 met17::ADE2 RAD5 This study
LSY842 MAT a rad53-21 met17-s ADE2 RAD5 This study
LSY843 MAT a rad53-21 met17::ADE2 RAD5 This study
LSY838 MAT a rad57::LEU2 met17-s ADE2 RAD5 This study
LSY839 MAT a rad57::LEU2 met17::ADE2 RAD5 This study
LSY837 MAT a rad59::LEU2 met17-s ADE2 RAD5 This study
LSY836 MAT a rad59::LEU2 met17::ADE2 RAD5 This study
LSY841 MAT a rad51::HIS3 rad59::LEU2 met17-s ADE2 RAD5 lys2 This study
LSY840 MAT a rad51::HIS3 rad59::LEU2 met17:: ADE2 RAD5 lys2 This study
  • a All strains are derivatives of W303 (leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 MET17 rad5-535). Only differences in the genotype from the parental strains are indicated, except for B404-4D, which is alsoade2::hisG:URA3:hisG his3::ade2-5′Δ:TRP1:ade2-n.