Table 2.

Effect of MafK overexpression on the α1 globin promoter activities in K562 cellsa

Reporter plasmidHS-40Cotransfected MafKMean relative promoter activityb ± SD (n)
pHS-40-α590GHWild type100 (8)
pHS-40-α590GHWild type+32 ± 1 (8)
pHS-40(3′-NA-II)-α590GH3′-NA(II)75 ± 12 (9)
pHS-40(3′-NA-II)-α590GH3′-NA(II)+83 ± 10 (9)
  • a K562 cells were first transiently cotransfected with pHS40-α590GH plus 1, 5, 10, 20, and 30 μg of pRSV-MafK, which resulted in promoter activities, as percentages of the activity of pHS-40-α590GH without cotransfected pRSV-MafK of 74 ± 7, 43 ± 11, 33 ± 11, 35 ± 10, and 26 ± 2, respectively. Two independent experiments were performed in duplicate.

  • b Effect of 30 μg of cotransfected pRSV-MafK on activities of the α1 globin promoter linked in cis to wild-type HS-40 or HS-40 with the 3′-NA(II) mutation. The promoter strengths are relative to that of pHS-40-α590GH without cotransfected pRSV-MafK.