Table 1.

Abp1p shows an intermediate binding constant compared to other DNA-binding proteins

ProteinKapp(M−1)aReference
Yeast Cbf1p3 × 108 b 4
Human CENP-B6 × 108 b 48
Xenopus 5S rRNA TFIIIA1 × 109 25
Fission yeast Abp1p7 × 109 b This study
Adenovirus major late TF1 × 1010 12
Yeast transcription factor tau5 × 1010 b 3
Human TFIIIC2 × 1011 b 7
  • a Apparent equilibrium binding constant.

  • b The apparent equilibrium constant was determined by the electrophoretic mobility shift method (3, 7). The value was corrected for the presence of nonspecific DNA in the binding reaction, yielding a specific equilibrium binding constant.