Table 1.

S. cerevisiae strains used in this study

StrainGenotypeSource
W303-1a MAT a ade2-1 ura3-1 his3-1 trp1-1 leu2-3,112 can1-100 R. Rothstein
YB0298W303-1a; cdc45-1 This laboratory
YB0556W303-1a; cdc7-1 This work
YB0557W303-1a; cdc7-4 This work
YB0349W303-1a; dbf4-1 This laboratory
YB0469W303-1a;cdc45::CDC45-HA3-LEU2 This laboratory
YB0476 MATα mcm2-1 cdc45::CDC45-HA3-LEU2 This laboratory
YB0549 MAT a rfa2-2 cdc45::CDC45-HA3-LEU2 This work
YB0472W303-1a; cdc7-1 cdc45::CDC45-HA3-LEU2 This laboratory
YB0547W303-1a; cdc7-4 cdc45::CDC45-HA3-LEU2 This work
YB0548W303-1a; dbf4-1 cdc45::CDC45-HA3-LEU2 This work
YB0576 MAT a ars1T860G cdc45::CDC45-HA3-LEU2 This work
YB0577 MAT a ars1::835-842 cdc45::CDC45-HA3-LEU2 This work
YB0578 MAT a ars1::798-805 cdc45::CDC45-HA3-LEU2 This work
YB0579 MAT a ars1::756,758 cdc45::CDC45-HA3-LEU2 This work
YB0553 MAT a ura3::GAL1-SIC1ΔNT-URA3 cdc45::CDC45-HA3-LEU2 This work
YB0477W303-1a; clb5::URA3 clb6::LEU2 cdc45::CDC45-HA3-LEU2 This laboratory
OAy618W303-1a;polɛ::POLɛ-HA3-LEU2 S. Bell
YB0550W303-1a; cdc45::CDC45-MYC3-LEU2 polɛ::POLɛ-HA3-LEU2 This work
YB0551 MAT a mcm2-1 cdc45::CDC45-MYC3-LEU2 polɛ::POLɛ-HA3-LEU2 This work
K6388W303-1a; leu2::DBF4-MYC18-LEU2 K. Nasmyth
YB0552W3031a; cdc45-1 leu2::DBF4-MYC18-LEU2 This work