Table 1.

S. cerevisiae strains used

StrainRelevant genotypeSource or reference
1783 MAT a a I. Herskowitz
1784 MATαa I. Herskowitz
1788 MAT a /MATαa I. Herskowitz
DL251 MAT a /MATα bck1Δ::URA3/bck1Δ::URA3a 25
DL376 MAT a pkc1Δ::LEU2a 26
DL1985 MAT a wsc1Δ::LEU2a 40
DL1986 MATα wsc1Δ::LEU2a 40
DL1987 MAT a /MATα wsc1Δ::LEU2/wsc1Δ::LEU2a 40
DL2069 MAT a /MATα rom2Δ::URA3/rom2Δ::URA3a This study
DL2276SFY526b Clontech Laboratories
DL2278 MAT a mid2Δ::URA3a 40
DL2282 MAT a mid2Δ::URA3 wsc1Δ::LEU2a 40
DL2393 MAT a cdc28-13 (D13au)c 40
DL2394 MAT a /MATα mid2Δ::URA3/mid2Δ::URA3a 40
DL2407 MAT a d W. Tanner
DL2408 MAT a pmt2Δ::LEU2d W. Tanner
DL2435 MAT a cdc28-13 mid2Δ::URA3c 40
DL2440 MAT a cdc28-13 pmt2Δ::LEU2c This study
DL2468 MAT a pmt2Δ::LEU2a This study
DL2550 MAT a wsc1Δ::LEU2 pmt2-1a This study
  • a EG123 background: leu2-3,112trp1-1 ura3-52 his4 can1.

  • b MATa ura3-52 his3-200 ade2-101 lys2-801 trp1-901 leu2-3, 112 Canr gal4-542 gal80-538 URA3::GAL1-lacZ.

  • c D13au background: ade1 his2 leu2-3, 112 trp1-1a ura3ΔNS.

  • d SEY6211 background: leu2-3, 112 ura3-52 his3-Δ200 ade2-101 trp1-Δ901 suc2-Δ9.