Table 1.

Microtargeting of alfalfa seedlings withenod40 DNA constructs

ConstructNo. of fociaNo. of roots examinedfdiv ± SEb
pDH511640.02 ± 0.02
p35S-Mtenod40 18350.51 ± 0.09
p35S-Mtenod40-Δ78200.40 ± 0.11
p35S-Mtenod40-Δ511300.37 ± 0.09
p35S-Mtenod40-Δ7-ACG sORF I1380.03 ± 0.03
p35S-Mtenod40-Δ5-ACG sORF II1150.07 ± 0.07
p35S-Mtenod40-Δ43200.15 ± 0.08
p35S-Mtenod40-Δ80200.00 ± 0.00
p35S-Mtenod40-Δ126120.50 ± 0.15
p35S-Mtenod40-ACG sORF I4460.09 ± 0.04
p35S-Msenod40-ACG sORF I190.11 ± 0.11
p35S-Mtenod40-TCA sORF I2330.06 ± 0.04
p35S-Mtenod40-mod.sORF I8270.30 ± 0.09
p35S-SoyMtenod40 1150.07 ± 0.07
p35S-Mtenod40-ACG sORF II2150.13 ± 0.09
p35S-Mtenod40-ΔRNA2150.13 ± 0.09
  • a Number of cortical cell division foci observed 2 days after microtargeting.

  • b Number of cortical cell division foci per bombarded root.