Table 3.

plc1Δ cells display mitotic-delay morphology

Strain% of cells with cell morphologya:
UBSBLBDB
Single nucleusTwo separate nuclei
Wild type37 ± 4.232 ± 4.012 ± 1.919 ± 2.50
plc1Δ25 ± 3.519 ± 2.126 ± 3.122 ± 2.78 ± 1.5
  • a Logarithmic asynchronous cultures of wild-type (W303-1a) and plc1Δ (Hl1-1) cells growing in YPD at 28°C were stained with 4′,6-diamidino-2-phenylindole, and nuclear and bud morphologies were scored. At least 300 separated cells were scored for each sample in each experiment. The experiment was performed three times, and standard deviations are indicated. UB, unbudded cells; SB, small-budded cells (with the diameter of the bud being less than 75% of the diameter of the mother cell); LB, large-budded cells with single nuclei (cells with single nuclei positioned within 50% of the mother cell proximal to the neck and with the diameter of the bud being at least 75% of the diameter of the mother cell); LB, large-budded cells with two separate nuclei (one nucleus in the mother cell, one nucleus in the bud); DB, cells with two buds (occasionally cells with more than two buds were observed and were also scored as DB).