Table 1.

Yeast strains used in this study

StrainGenotypeSource and/or reference
W303-1a MAT a ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 ssd1-d2 can1-100 R. Rothstein
YPH499 MAT a ade2-101 his3-Δ200 leu2-Δ1 lys2-801 trp1-Δ63 ura3-52 P. Hieter
PM1201 MATα his3-Δ200 leu2-3,112 trp1 ura3-52 CSE4-HA M. M. Smith; 46
HL1-1W303-1aplc1::URA3 41
PN101YPH499 plc1::URA3 This study
CG1945 MAT a ura3-52 his3-200 lys2-801 trp1-901 ade2-101 Leu2-3,112 gal4-542 gal80-538 LYS::GAL1-HIS3 Cyhr2 URA3::GAL4 17-mers(×3) -CYC1-lacZ Clontech
YM4271 MAT a ura3-52 his3-200 lys2-801 ade2-101 ade5 trp1-901 leu2-3,112 tyr1-501 gal4Δ gal80Δ ade5::hisG Clontech
JC107YM4271[pHISi-1-CEN]This study
JC108YM4271[pHISi-1-CEN M53]This study