Table 2.

Effect of PLC1 mutations on the mitotic stability of minichromosomes

StraineGeneration timedAvg loss per generation (%)a with:
pRS314b plus nocodazole at:pUN20c
0 μg/ml2 μg/ml
PLC1 1.51.0 ± 0.11.9 ± 0.21.4 ± 0.1
plc1Δ 5.06.1 ± 0.832.4 ± 4.56.1 ± 0.6
plc1-8 4.03.1 ± 0.412.7 ± 1.63.5 ± 0.4
plc1-29 2.52.1 ± 0.34.1 ± 0.41.9 ± 0.2
plc1-39 3.02.5 ± 0.35.9 ± 0.71.3 ± 0.1
  • a The minichromosome stability of pRS414 or pUN20 was measured for at least five independent transformants and is reported as the percentage of minichromosome loss per generation at 28°C (standard deviations are also indicated).

  • b The mitotic stability of the pRS413 minichromosome was measured as a fraction of cells that retained the minichromosome after growth in nonselective medium (35, 43) as described in Materials and Methods. All strains used for this assay were derived from W303-1a.

  • c The mitotic stability of the pUN20 minichromosome was measured by using the half-sectored colony assay (27) as described in Materials and Methods. All strains used for this assay were derived from YPH499.

  • d Generation time (in hours) was measured in YPD medium at 28°C for strains derived from W303-1a.

  • e PLC1, plc1Δ, plc1-8,plc1-29, and plc1-39 denote plc1Δ strains harboring the pRS413-PLC1, pRS413, pRS413-plc1-8, pRS413-plc1-29, and pRS413-plc1-39 plasmids, respectively.