Table 1.

Half-lives of β-globin Cox-2 reporter mRNAs in the presence of MKK6, dexamethasone, or SB203580a

Transcript Half-lifeb of transcript (h)
Control MKK6 MKK6+Dex MKK6+SB
β-globin–Cox2.5 1.16 2.00 1.08 1.32
β-globin–Cox1.4 1.24 2.06 1.29 1.07
β-globin–Cox0.6 1.02 2.24 1.31 1.15
β-globin–Cox0.1 1.26 4.16 1.65 1.64
β-globin–Cox0.5NMc NM NMNM
  • a Transfections, treatments, and RNase protection assays were essentially as described in the legend to Fig.2. SB203580 (1 μM) was added 30 min prior to the addition of tetracycline, and dexamethasone (1 μM) was added 2 h prior to the addition of tetracycline.

  • b Half-lives were calculated from plots of β-globin/GAPDH ratio against time. The half-lives shown are from two representative experiments.

  • c NM, not measurable.