Table 2.

Plasmids and oligonucleotides used in this study

Plasmid or oligonucleotideDescription or sequenceReference
Plasmids
 pPL1935-kbEcoRI fragment containing PTR3 in pRS31634
 pPL341 ptr3Δ14::hisG-URA3-kanr-hisGin pUC118 (ΔB-ΔH)34
 pPL3563.9-kbSpeI-ClaI fragment containing SSY1 in pRS31634
 pPL4967.8-kb Sau3A fragment containing SSY5 in pRS202This work
 pHK003pPL356 with all XbaI sites removed34
 pHK010 SSY1-HA1 in pRS31634
 pHK012 SSY1 in pRS202This work
 pHK018 PTR3-HA1 in pRS31634
 pHK026 PTR3 in pRS202This work
 pHK031 ssy1Δ12::hisG-URA3-kan r-hisGin pBluescriptThis work
 pHK0354.9-kbBstXI-BamHI fragment containing SSY5in pRS316This work
 pHK036Same as pHK035, exceptSSY5 fragment inserted in opposite orientationThis work
 pHK037 SSY5 in pRS202This work
 pHK038 SSY1-NT(1–206) in pRS316This work
 pHK039 SSY1-NT(1–206) in pRS202This work
 pHK040pPL193 with BamHI site in MCS removedThis work
 pHK041pHK036 with BamHI site in MCS removedThis work
 pHK042pHK040 withBamHI site (b) introduced after ATG inPTR3 This work
 pHK043pHK041 withBamHI site (b) introduced after ATG inSSY5 This work
 pHK044 PTR3 in pSOS This work
 pHK045 SSY5 in pSOSThis work
 pHK047pHK041 with BamHI site (a) introduced after the ATG of SSY5 ORFThis work
 pHK048 SSY5-c-myc in pRS316This work
 pHK049 ssy5Δ1::hisG-URA3-kan r-hisGin pRS316This work
 pHK050 SSY5 in pAD40This work
Oligonucleotides
 POL95-0395′-GATTGAGTTGAATTCTTCTACCACTAC-3′
 POL96-0215′-CCTGGCTGATTTCTAGATAGGGTTATATG-3′
 POL99-0265′-GATATACCGATGGGATCCGTCAGATTTTTTGG-3′
 POL00-0045′-GATATACCGATGGGGATCCTCAGATTTTTTGG-3′
 POL00-0055′-TAGGCGTTCATGGGGATCCACTCTCATAG-3′