Table 1.

Oligonucleotides used in the present study

Primer5′-end coordinateaDNA sequenceAnnealing temp (°C)
RA-150305′-CGATAACGAACGAGACTCTGG60.0
RA-250495′-GGCATGCTAACTAGTTACGCGACCCC72.0
RA-350595′-CTAGTTACGCGACCCCCGAGCG70.0
RC-155415′-CCTCCGGGCTCCGTTAATG56.0
RC-255235′-GATCCTTCCGCAGGTTCACC64.0
RC-355175′-TCCGCAGGTTCACCTACGGAAACC69.0
RD-152945′-CTCGTTCATGGGGAATAATTG54.0
RD-252755′-TGCAATCCCCGATCCCCATCA64.0
RD-352705′-TCCCCGATCCCCATCACGAATGG64.0
RF-1120645′-CGCTCCGGGGACAGTGCCAG62.0
RF-2120835′-GGTGGGGAGTTTGACTGGGGCGGTAC72.0
RF-3120915′-GTTTGACTGGGGCGGTACACCTGTC72.0
RG-1126425′-GGCGCTGCCGTATCGTTCGC62.0
RG-2126235′-CCTGGGCGGGATTCTGACTTAGAGG72.0
RG-3126165′-GGGATTCTGACTTAGAGGCGTTCAGTC72.0
LinkerLinker5′-GCGGTGACCCGGGAGATCTGAATTC CTAGACTTAAG-5′72.0