Table 1.

Yeast strains

StrainGenotypeSource
W303 MAT a ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 canR1-100 48
W303ΔRIP1 MAT a ΔRIP1::KANr; otherwise isogenic to W303 48
rna1-1 MAT a ura3-52 leu2Δ1 trp1 rna1-1 3
prp20-1 MATα ura3-52 leu2Δ1 trp1Δ63 prp20-1 21
prp20-7 MAT a ura3-52 his3Δ200 tyr1 ade2-1 prp20-7 39
prp20-101 MAT a ura3-52 leu2Δ1 his3Δ200 Gal+ prp20-101 51
ΔYRB2 MAT a ura3 leu2 ade2 trp1 YRB2::HIS3 51
mex67-5 MAT a ade2 leu2 ura3 trp1 MEX67::HIS3 pRS314-TRP1-mex67-5 41
mtr2-9 MAT a ade2 leu2 ura3 trp1 MTR2::HIS3 pRS315-LEU2-mtr2-9 41
xpo1-1 MATα ade2 his3 trp1 ura3 can1 XPO1::LEU2 pKW457-HIS3-xpo1-1 45
PSY580 MAT a ura3-52 trp1Δ63 leu2Δ1 Gal+ 42
pse1-1 MAT a ura3-52 trp1Δ63 leu2Δ1 Gal+pse1-1 42
ΔKAP123 MATα ura3-52 leu2Δ1 KAP123::HIS3 42
ΔSXM1 MAT a ura3-52 leu2Δ1 trp1Δ63 ADE+SXM1::HIS3 42
rat8-2 (or dbp5-2) MAT a leu2Δ1 trp1Δ63 ura3-52 rat8-2 44
gle1-8 MAT a ade2 his3 leu2 trp1 ura3 gle1-8 F. Stutz
rat7-1 (or nup159-1) MAT a trp1Δ63 ura3-52 leu2Δ1 rat7-1 10
ΔNUP133 MATα ade2 his3 leu2 trp1 ura3 NUP133::HIS3 5
ΔNUP100 MAT a ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup100-3::TRP1 52
ΔNUP116 MAT a ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup116-5::HIS3 52
ΔNUP145 MAT a ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup145-1::URA3 52
NUP82Δ108 MAT a ade2 leu2 ura3 trp1 NUP82::HIS3 pRS316-URA-NUP82Δ108 16
nup49-313 MATα ade2 ade3 his3 leu2 ura3 NUP49::TRP1 pUN100-LEU2-nup49-313 5
ΔNUP57 MAT a ade2 leu2 ura3 trp1 NUP57::HIS3 E. Hurt, V. Doye
rpb1-1 MATα leu2 ura3 Gal+ rpb1-1 R. Young