Table 1.

Plasmids and yeast strains used

Strain or PlasmidRelevant features or genotypeSource or reference
Plasmids
 pBM272 ampR URA3 CEN4 ARS1 pGAL1 pGAL10 33
 pCG01pBM272pGAL10-INT1 23
 pCG110pCG01ura3::LEU2 This study
 pCG108pCG108ura3::TRP1 This study
 pYES2 ampR URA3 2μm oripGAL1 Invitrogen, Inc.
 pYES2-CaSLA2 pYES2 pGAL1-CaSLA2 This study
S. cerevisiae
 Actin charge-to-alanine scanning mutants
  YJB2603 MAT a ACT1::HIS3 his3-Δ200 leu2-3,112 ura3-52 ade4 tub2-201 [pCG01] 67
  YJB2604YJB2603 act1-101::HIS3 can1 [pCG01] 67
  YJB2606YJB2603act1-113::HIS3 can1[pCG01] 67
  YJB2607YJB2603act1-119::HIS3[pCG01] 67
  YJB2608YJB2603act1-120::HIS3[pCG01] 67
  YJB2609YJB2603act1-122::HIS3[pCG01] 67
  YJB2610YJB2603act1-124::HIS3[pCG01] 67
  YJB2611YJB2603act1-129::HIS3[pCG01] 67
  YJB2612YJB2603act1-133::HIS3[pCG01] 67
  YJB2614YJB2603act1-104::HIS3[pCG01] 67
  YJB2615YJB2603act1-115::HIS3[pCG01] 67
  YJB2617YJB2603act1-117::HIS3[pCG01] 67
  YJB2618YJB2603act1-123::HIS3[pCG01] 67
Actin cytoskeleton mutants
  YJB2714 MATα ABP1 ura3-52 leu2-3[pCG01] 69
  YJB2715 MAT a abp1::LEU2 ura3-52 lys2-801 am leu2-3,112 [pCG01] 69
  YJB3054 MAT a SAC6 his3 leu2 lys2 ura3 [pCG01]A. Adams
  YJB3053YJB3054sac6::LEU2 [pCG01]A. Adams
  YJB3321 MAT aBNI1/BNI1 DFG9/DFG9 SRV2/SRV2 ura3-52/ura3-52 [pCG01] 41
  YJB3331 MAT a/MATα bni1-100/bni1-100 ura3-52/ura3-52 leu2::hisG/leu2::hisG trp1::hisG/TRP1 [pCG01] 53
  YJB3329 MAT a/MATα srv2-100/srv2-100 ura3-52/ura3-52 leu2::hisG/leu2::hisG trp1::hisG/TRP1 [pCG01] 53
  YJB2457 MAT a/MATα dfg9-100/dfg9-100 ura3-52/ura3-52 leu2::hisG/leu2::hisG trp1::hisG/TRP1 [pCG01] 53
  YJB3057 MATα RVS167 his3Δ200 leu2-3,112 ura3-52 trp1-1(am) lys2-801(0c) [pCG01] 39
  YJB3056 MATα rvs167::TRP1 his3Δ200 leu2-3,112 ura3-52 trp1-1(am) lys2-801(0c)[pCG01] 39
  YJB2623 MAT a VRP1 ura3-52 ade1 leu2-3,112 ile MEL1 [pCG01]A. Hopper
  YJB2622 MATα vrp1::LEU2 leu2-3,112 ade1 ura3-52 ile MEL1 [pCG01] 72
  YJB3156 MAT a SLA1 SLA2 leu2-3,112 ura3-52 [pCG110] 28
  YJB3155 MAT a sla1-Δ1::URA3 leu2-3,112 ura3-52[pCG110] 28
  YJB3157 MATα ura3-52 leu2-3,112 sla2Δ::URA3[pCG110] 69
  YJB2489 MAT a ura3-52 leu2-Δ2 his3-Δ200 trp1-Δ63 lys2-801 Mark Longtine
  YJB4786YJB2489sla2::HIS3 This study
cdc3 strains
  YJB2757 MATα ura3-52 leu2-Δ2 his3-Δ200 trp1-Δ63 lys2-801(SLA2) 9
  YJB3857YJB2757sla2::HIS3 This work
  YJB5565 MAT a ura3-52 leu2-Δ2 his3-Δ200 trp1-Δ63 lys2-801 cdc3-6 This work
  YJB5566 MAT a ura3-52 leu2-Δ2 his3-Δ200 trp1-Δ63 lys2-801 cdc3-6 sla2::HIS3 This work
Sla2/End4 domain deletion strains
  YJB4553 MAT a/MATα SLA2/SLA2 ura3-52/ura3-52 leu2-3,112/leu2-3,112 lys2-801/lys2-801 his3-Δ200/his3-Δ200/his3-Δ200 ade2-1/ADE2[pCG110] 70
  YJB5176YJB4553sla2Δ::URA3/sla2Δ::URA3[pCG110] 70
  YJB4550YJB4553sla2Δ768-968::URA3/sla2Δ768-968::URA3[pCG110] 70
  YJB4549YJB4553sla2Δ501-968::URA3/sla2Δ501-968::URA3[pCG110] 70
  YJB4552YJB4553sla2Δ360-968::URA3/sla2Δ360-968::URA3[pCG110] 70
  YJB5163 MAT a sla2-Δ1::URA3 his3-Δ200 leu2-3,112 ura3-52 [pCG110, pRS313] 70
  YJB5164YJB5163 [pCG110, pDD353(SLA2)] 70
  YJB5165YJB5163 [pCG110, pDD362 (sla2Δ33-750)] 70
  YJB5166YJB5163 [pCG110, pDD364(sla2Δ33-501)] 70
  YJB5167YJB5163 [pCG110, pDD367 (sla2Δ33-359)] 70
  YJB5168YJB5163 [pCG110, pDD369(sla2Δ33-359, 576 stop)] 70
  YJB5169YJB5163 [pCG110, pDD371 (sla2Δ360-575)] 70
  YJB3149 MAT a his3 lys2 ura3 leu2 bar1 end4::HIS3 END4:TRP1[pCG110] 68
  YJB3146YJB3149end4Δ114-284:TRP1 [pCG110] 68
  YJB3154YJB3149 end4Δ286-301:TRP1[pCG110] 68
  YJB3147YJB3149end4Δ318-373:TRP1[pCG110] 68
  YJB3150YJB3149end4Δ376-501:TRP1 [pCG110] 68
  YJB3151YJB3149 end4Δ495-573:TRP1[pCG110] 68
  YJB3152YJB3149end4Δ376-573:TRP1 [pCG110] 68
  YJB3153YJB3149 end4Δ376-440:TRP1[pCG110] 68
  YJB3148YJB3149end4Δ767-968:TRP1 [pCG110] 68
Strains used in complementation studies
  YJB4686 MAT a lys2 his4 leu2 ura2 bar1 end4 sla2::LEU2 GAL H. Reizman
  YJB4896YJB2757 [pCG108, pYES-CaSLA2]This study
  YJB4897YJB3857 [pCG108, pBM272]This study
  YJB4897YJB3857 [pCG108, pYES2]This study
  YJB4898YJB3857 [pCG108, pYES-SLA2]This study
C. albicans strains
  YJB1873 (CAF2) ura3::imm434/URA3 CaSLA2/CaSLA2 21
  YJB3018 (CAI4) ura3::imm434/ura3::imm434 CaSLA2/CaSLA2 21
  YJB3400YJB3018Casla2::hisG::URA3::hisG/CaSLA2 This study
  YCA37YJB3018Casla2::hisG::URA3::hisG/CaSLA2 This study
  YJB3401YJB3400Casla2::hisG/CaSLA2 This study
  YJB3611YCA37Casla2::hisG/CaSLA2 This study
  YJB3402YJB3401Casla2::hisG/Casla2::hisG::URA3::hisG This study
  YJB3612YJB3611Casla2::hisG/Casla2::hisG::URA3::hisG This study