Table 1.

Phenotypes of progeny from crosses between UAS-dSF2/ASF or UAS-hSF2/ASF transgenic lines and GMR-GAL4 line reared at different temperaturesa

Genotype of transgenic flies overexpressing:Temp (°C)Observed phenotype (relative strength of retina defects)
dSF2/ASF
W1118; UAS-dASF 1/GMR-GAL4 22Adults of both sexes with retina defects (++)
25No adult males; adult females with retina defects (+++)
28Late pupal lethality
W1118; UAS-dASF2 2/GMR-GAL4 22Adults of both sexes with retina defects (+++)
25No adult males; adult females with retina defects (+++)
28Late pupal lethality
W1118; GMR-GAL4/+; UAS-dASF2 3/+22Adults of both sexes with retina defects (+)
25Adults of both sexes with retina defects (++)
28Late pupal lethality
W1118; GMR-GAL4/+; UAS-dASF2 4/+22Adults of both sexes with retina defects (++)
25Adults of both sexes with retina defects (+++)
28Late pupal lethality
W1118;GMR-GAL4/+; UAS-dASF2 5/+22Adults of both sexes with retina defects (+)
25Adults of both sexes with retina defects (++)
28Late pupal lethality
W1118; GMR-GAL4/+; UAS-dASF2 6/+22Adults without apparent abnormalities
25Adults of both sexes with retina defects (+)
28Adults of both sexes with retina defects (++)
W1118; UAS-dASF2 7/GMR-GAL4 22Adults of both sexes with retina defects (++)
25Rare adult males; all adults with retina defects (+++)
28Late pupal lethality
hSF2/ASF
W1118; UAS-hASF2 1/GMR-GAL4 22Adults of both sexes with retina defects (+)
25Adults of both sexes with retina defects (++)
28No males; adult females with retina defects (+++)
W1118; UAS-hASF2 2/GMR-GAL4 22Adults of both sexes with retina defects (+)
25Adults of both sexes with retina defects (++)
28Late pupal lethality
W1118;GMR-GAL4/+; UAS-hASF2 3/+22Adults of both sexes with retina defects (+)
25Adults of both sexes with retina defects (++)
28No males; adult females with retina defects (+++)
W1118;GMR-GAL4/+; UAS-hASF2 4/+22Adults without apparent abnormalities
25Adults of both sexes with retina defects (+)
28Adults of both sexes with retina defects (+++)
W1118; UAS-hASF2 5/GMR-GAL4 22Adults without apparent abnormalities
25Adults of both sexes with retina defects (+)
28No males; Adult females with retina defects (+++)
W1118; GMR-GAL4/+;UAS-hASF2 6/+22Adults of both sexes with retina defects (+)
25Adults of both sexes with retina defects (++)
28No males; adult females with retina defects (+++)
W1118; UAS-hASF2 7/GMR-GAL4 22Adults without apparent abnormalities
25Adults of both sexes with retina defects (+)
28No males; adult females with retina defects (+++)
  • a More than 100 progeny were examined.