Table 2.

Some actin alleles affect INT1-induced filamentous growth

Actin alleleaAmino acids changed to alaninebActin subdomain mutatedcINT1-induced filamentous growthdFilamentous colony growtheBud site selection patternfSynthetic lethality withg
ACT1 ++++++ Unipolar
Temperature sensitivity
act1-101 363, 3641+++ sla2Δ
act1-113 210, 2114 ++++++Unipolar sac6Δ
act1-119 116, 117, 1181+++ sac6Δ
act1-120 99, 1001++Random sla2Δ
act1-122 80, 812+++
act1-124 56, 572+Random sla2Δ, sac6Δ
act1-129 177, 1793Random sla2Δ, sac6Δ, abp1Δ
act1-133 24, 251+++ sla1Δ, sla2Δ
Pseudo-wild type
act1-104 315, 3163+++Unipolar
act1-115 195, 1964+++ sla2Δ, sac6Δ
act1-117 183, 1844+++++Bipolar
act1-123 68, 722+++
  • a Strains used were YJB2603 to YJB2618, as listed in Table 1.

  • b Phenotypes are as described by Wertman et al. (67).

  • c Domains are as assigned by Botstein et al. (11).

  • d Proportion of cells forming elongated filamentous cells. +++, like wild type; ++, 25 to 75% of wild-type levels; +, 5 to 25% of wild-type levels; −, less than 2% of wild-type levels.

  • e Filamentous growth was described by Cali et al. (14). +++, like wild type; ++, 80 to 100% of colonies had filaments, but filaments were disorganized relative to the wild type; +, 1 to 10% of colonies had filaments; −, no filaments extending beyond the perimeter of the colony.

  • f Bud site selection was determined by Cali et al. (14).

  • g Data were reviewed by Botstein et al. (11).