Table 1.

Mutations in theHindIII-HpaI‚ÄČregion

MutationWild-type sequenceMutant sequenceNew restriction site
M1TTTCCTATAGTTGGGATCCGCTGG BamHI
M2GAAAAGCTCAAGGCCTAGAG StuI
M3AGAGAATTGACTCTCCGGAC BspEI
M4GTATAAGTTTTGCGCCCGGG SmaI
M5ATGAGTGCTTCGTCCCATGG NcoI
M6AGCGGCAAACCTATTGGCCA MscI
M7GCACCATCAGTACGTCGACT SalI
M8AGTTTTTTCCCCGGGGGGAA SmaI
M9TTCATGGAGCGCACGTTCTA FspI
M10GACAGTTTGCTCACTGGGAA EcoRI
M11GATTTGCCCGACGCGTAAAT MluI
M12AGCGTGAAAGCTATGTTCTA XbaI
M13AAGCTTCCCGCCATGGAAAT NcoI