Table 2.

Overexpression of UPF1, NMD2, orUPF3 in wild-type andupf/nmd strainsa

StrainCanavanine sensitivity (μg/ml)
YEpYEp-UPF1YEp-NMD2YEp-UPF3
Wild type300300300300
upf1Δ253002525
nmd2Δ357530035
upf3Δ35–407525300
upf1Δ nmd2Δ 25752525
upf1Δ upf3Δ 25752525
nmd2Δ upf3Δ 35753535
upf1Δ nmd2Δ upf3Δ 501255050
  • a Suppression assays are summarized, with the concentration of canavanine at which each strain began to exhibit the canavanine-sensitive phenotype indicated (canavanine sensitivity). Yeast strains of the indicated genotypes were transformed with the high-copy-number vector alone (YEp; control) or high-copy-number plasmids expressing UPF1, NMD2, andUPF3 (YEp-UPF1, YEp-NMD2, and YEp-UPF3, respectively).