Table 2.

Overproduction of Red1 or Mek1 promotes sporulation of the zip1 mutant

StrainMutant genotypeSporulationa (%)Nuclear divisionb (%)Spore viabilityc (%)
JM474Wild type + YEp35266.767.998.0%
JM153 zip1 + YEp352<1.0<1.0 NDd
JM107e zip1 + YEp24<1.0<1.0ND
JM155 zip1 + pB86 (YEp352RED1)66.266.72.7
JM76 zip1 + pNH83-2 (YEp24HOP1)<1.0<1.0ND
JM154 zip1 + pB133 (YEp352MEK1)28.028.215.9
JM64 zip1 + pJ14 (YEp352 MEK1 RED1)<1.0<1.0ND
JM65 zip1 + pJ70 (YEp352 MEK1 HOP1)<1.0<1.0ND
JM106 zip1 + pJ66 (YEp352 RED1 HOP1)<1.0<1.0ND
  • a Includes dyads, triads, and tetrads, measured after 72 h in sporulation medium.

  • b Cells that have undergone meiosis I or meiosis I and II after 72 h in sporulation medium.

  • c Based on dissection of 70 tetrads per strain.

  • d ND, not determined.

  • e Strain JM107 is the control for strain JM76; strain JM153 is the control for strains JM155, JM154, JM64, JM65, and JM106.