Table 4.

Overproduction of Red1 decreases gene conversion

StrainMutant genotypePrototrophsa
HistidineThreonineTryptophan
JM117 spo13 + YEp352c 41.0 2.2 1.9 
JM116 spo13 + pB86 (YEp352RED1)22.0 (1.8)b 0.9 (2.4)1.0 (1.9)
JM115 spo13 + pB133 (YEp352MEK1)39.0 (1.0)2.2 (1.0)1.8 (1.0)
JM119 spo13 + YEp2437.0 2.4 2.2 
JM118 spo13 + pNH83-2 (YEp24HOP1)28.0 (1.3)1.6 (1.5)1.5 (1.5)
JM112 spo13 zip1 + YEp35236.4 1.7 6.1 
JM111 spo13 zip1 + pB86 (YEp352RED1)6.2 (5.6)0.4 (3.6)1.5 (4.7)
JM110 spo13 zip1 + pB133 (YEp352MEK1)35.5 (1.0)1.6 (1.1)4.3 (1.4)
JM212 spo13 zip1 + pJ14 (YEp352 MEK1 RED1)25.2 (1.4)1.1 (1.5)3.8 (1.6)
JM213 spo13 zip1 + pJ70 (YEp352 MEK1 HOP1)38.6 (0.94)1.3 (1.3)4.4 (1.4)
JM214 spo13 zip1 + pJ66 (YEp352 RED1 HOP1)31.3 (1.2)1.3 (1.3)4.7 (1.3)
JM113 spo13 zip1 + YEp2435.0 1.7 7.0 
JM114 spo13 zip1 + pNH83-2 (YEp24HOP1)23.3 (1.5)1.2 (1.4)4.6 (1.5)
  • a Cells returned to growth after 72 h in sporulation medium. Values indicated are frequencies per 104 cells.

  • b Values in parentheses indicate the fold decrease relative to that of the appropriate control strain.

  • c Strain JM117 is the control for JM116 and JM115; strain JM119 is the control for JM118; strain JM112 is the control for JM111, JM110, JM212, JM213, and JM214; and strain JM113 is the control for JM114.