TABLE 1

Human primers used for real time RT-PCR

ProteinPrimer nucleotide sequencesaProduct size (bp)
Runx2FOR, 5′-GGCCCTGGTGTTTAAATGGT-3′; REV, 5′-AGGCTGTTTGACGCCATAGT-3′178
ALPFOR, 5′-CTGCAAGGACATCGCCTATC-3′; REV, 5′-CATCAGTTCTGTTCTTGGGGTA-3′101
A2BARFOR, 5′-TCCATCTTCAGCCTTCTGGC-3′; REV, 5′-AAAGGCAAGGACCCAGAGGA-3′128
β-ActinFOR, 5′-GCACTCTTCCAGCCTTCCTTCC-3′; REV, 5′-GAGCCGCCGATCCACACG-3′254
  • a FOR, forward; REV, reverse.