Table 1.

Strains used in this study

Strain Genotypea
JKM146Δho Δhml::ADE1 MAT a-inc Δhmr::ADE1 ade1 leu2-3,112 lys5 trp1::hisG ura3-52 ade3::GAL::HO
tNR85Δho HML matΔ::LEU2 hmrΔ3 leu2-3,112 trp1 thr4 ura3-52 pFH800
tNS709tNR85 (THR4 ura3 [29 bp]-cut site-URA3)
tNS747tNR85 (THR4 ura3 [142 bp]-cut site-URA3)
tNS759tNR85 (THR4 ura3 [415 bp]-cut site-URA3)
tNS1103tNR85 (THR4 ura3 [205 bp]-cut site-URA3)
tNS1110tNR85 (THR4 ura3 [235 bp]-cut site-URA3)
tNS1142tNR85 (THR4 ura3 [63 bp]-cut site-URA3)
tNS1157tNR85 (THR4 ura3 [89 bp]-cut site-URA3)
tNS1379Δho HML matΔ::leu2::hisG hmrΔ3 leu2-3,112 ura3-52 trpl thr4 (ura3 [205 bp]-cut site-URA3) pFH800
tNS1530tNR85 (THR4 ura3 [1,170 bp]-cut site-URA3)
tNS1551tNS759msh3::KanMX
tNS1573tNS1379rad59::KanMX
tNS1585tNS1379srs2::KanMX2
tNS1597tNS1379rad51::LEU2
tNS1703JKM146rad59::KanMX2
tNS1706JKM146msh3::LEU2 rad59::KanMX2
tNS1712tNS1530 rad59::KanMX2
tNS1785tNS759 rad59::KanMX2
tNS1853JKM146 srs2::LEU2 rad59::KanMX2
tNS1967tNS759rad59::NatMX4 msh3::KanMX
tNS1984tNS709 rad59::KanMX2
tNS1990tNS709 rad52::KanMX2
YFP218 (tNS1924)JKM146 srs2::LEU2
YFP259JKM146 msh3::LEU2
  • a Cut site refers to the 117-bpBglII-HincII MAT a HO endonuclease recognition site.