Table 1.

Yeast strains

StrainGenotypeReference or source
NDY341 MATα GAL1-STE3 ura3 leu2 his4 bar1-1 35
NDY342a MATα GAL1-STE3 end4-1 35
NDY343a MATα ste3Δ::LEU2 36
NDY344a MATα ste3Δ::LEU2 end4-1 36
NDY356a MATα GAL1-STE3 pep4Δ 35
NDY788a MATα GAL1-STE3 akr1Δ::LEU2 This work
NDY990a MATα GAL1-STE3-Ubiquitin(7K→R,G76A) 34
NDY991a MATα GAL1-STE3-Ubiquitin(7K→R, G76A)end4-1 This work
NDY992a MATα GAL1-STE3-Ubiquitin(7K→R, G76A)pep4Δ This work
NDY1011a MATα GAL1-STE3-Ubiquitin(7K→R, G76A)akr1Δ::LEU2 This work
NDY1040a MATα GAL1-STE3 vrp1Δ::URA3 This work
NDY1042a MATα GAL1-STE3-Ubiquitin(7K→R, G76A)vrp1Δ::URA3 This work
NDY1046a MATα GAL1-STE3 end3Δ::G418r This work
NDY1076a MATα GAL1-STE3-Ubiquitin(7K→R, G76A)end3Δ::G418r This work
LRB758 MAT a ura3-52 leu2 his3 28
LRB756b MAT a yck1-1::ura3yck2-2t s 28
LRB757b MATα STE3 yck1-1::ura3yck2-2t s 28
LRB759b MATα STE3 ura3-52 leu2 his3 28
NDY547b MATα ste3Δ::LEU2 This work
NDY548b MATα ste3Δ::LEU2 yck1-1::ura3 yck2-2t s This work
NDY662b MATα GAL1-STE3Δ365 yck1-1::ura3 yck2-2t s This work
NDY877b MATα GAL1-STE3 This work
NDY913b MATα GAL1-STE3 yck1-1::ura3 yck2-2t s This work
NDY1012b MATα GAL1-STE3-Ubiquitin(7K→R, G76A)This work
NDY1037b MATα STE3 akr1Δ::LEU2 This work
NDY1039b MATα GAL1-STE3Δ320-413 This work
NDY1045b MATα GAL1-STE3Δ320-413 yck1-1::ura3 yck2-2t s This work
NDY1061b MATα GAL1-STE3Δ320-413 akr1Δ::LEU2 This work
NDY1070b MATα GAL1-STE3Δ320-413 end3Δ::G418r This work
NDY1072b MATα GAL1-STE3Δ365 This work
NDY1074b MATα GAL1-STE3-Ubiquitin(7K→R, G76A)end3Δ::G418r This work
NDY1080b MATα GAL1-STE3 pep4Δ This work
NDY1083b MATα GAL1-STE3 akr1Δ::LEU2 This work
NDY1085b MATα GAL1-STE3-Ubiquitin(7K→R, G76A)akr1Δ::LEU2 This work
NDY1090b MATα GAL1-STE3-Ubiquitin(7K→R, G76A)yck1-1::ura3 yck2-2t s This work
NDY1117b MATα GAL1-STE3Δ365 end3Δ::G418r This work
NDY1118b MATα GAL1-STE3 end3Δ::G418r This work
NDY1215b MAT a akr1Δ::LEU2 This work
NDY1216b MATα GAL1-STE3Δ365 akr1Δ::LEU2 This work
NDY1218b MATα GAL1-STE3-Ubiquitin(7K→R, G76A)akr1Δ::LEU2 pep4Δ This work
NDY1223b MATα GAL1-STE3-Ubiquitin(7K→R, G76A)yck1-1::ura3 yck2-2t spep4Δ This work
NDY414 MATα HIS3::STE3 ura3 leu2 ade2-1o c ade1 his6 trp1a m 8
  • a Isogenic to NDY341.

  • b Isogenic to LRB758.