Table 3.

Metabolic characteristics of WT, PTP-1B+/−, and PTP-1B−/− mice fed either a chow or a high-fat dieta

ParameterChow dietHigh-fat diet
WTPTP-1B+/−PTP-1B−/−WTPTP-1B+/−PTP-1B−/−
Fed, serum leptin (ng ml−1)7.86 ± 1.16a 5.59 ± 0.41a,b 5.02 ± 0.42b 13.37 ± 2.57a 9.36 ± 2.32b 5.24 ± 1.15c
Serum FFA (mM)
 Fed0.90 ± 0.08a 0.89 ± 0.05a 0.88 ± 0.04a 0.54 ± 0.05a 0.53 ± 0.07a 0.63 ± 0.06a
 Fasted1.77 ± 0.14a ND1.46 ± 0.08a 1.31 ± 0.07a 1.55 ± 0.07a 1.42 ± 0.08a
Blood glucose (mg dl−1)
 Fed133 ± 4.73a 124 ± 4.35a 114 ± 2.54b 132 ± 4.62a 126 ± 5.60a,b 115 ± 3.50b
 Fasted67.7 ± 2.74a 62.2 ± 2.58a 57.5 ± 2.67b 79.9 ± 3.83a 77.3 ± 7.68a,b 66.6 ± 3.87b
Serum insulin (ng ml−1)
 Fed1.89 ± 0.28a 1.21 ± 0.09a,b 0.81 ± 0.10b 1.57 ± 0.22a 1.75 ± 0.18a 0.59 ± 0.09b
 Fasted0.36 ± 0.07a 0.25 ± 0.03a 0.27 ± 0.03a 0.50 ± 0.05a 0.53 ± 0.11a,b 0.35 ± 0.05b
  • a Male PTP-1B−/−, PTP-1B+/−, or WT mice were fed a chow or high-fat diet ad libitum. Serum was collected from fed or fasted mice between 8 and 10 weeks of age, and the indicated metabolic parameters were measured. Values are expressed as the mean ± SEM of measurements obtained for 6 to 10 animals per group. ND, not determined. Within a particular diet, values in the same row not sharing a superscript letter are significantly different (P < 0.05).