Table 1.

Plasmids used in this study

PlasmidaConstructionReference
pGAD backbone
 pSW1126Full-lengthNUP82 locus in BamHI siteThis study
 pSW1212Fragment encoding amino acids 1–187 of NUP82 locus inBamHI siteThis study
 pSW1213Fragment encoding amino acids 1–254 of NUP82 locus in BamHI siteThis study
 pSW1215Fragment encoding amino acids 1–409 of NUP82 locus inBamHI-PstIThis study
 pSW1217Fragment encoding amino acids 1–651 ofNUP82 locus in BamHI-PstIThis study
 pSW1218Fragment encoding amino acids 351–713 ofNUP82 locus in ClaI-BglIIThis study
 pSW1219Fragment encoding amino acids 482–713 ofNUP82 locus in ClaI-BglIIThis study
 pSW1216Full-length NSP1 locus inBamHI-PstIThis study
pGBT8 (GBD) backbone
 pSW259Fragment encoding amino acids 2–664 ofNUP145 locus inBamHI-SacI 11
 pSW413Full-length GLE1 locus inBamHIThis study
 pSW423Fragment encoding amino acids 590–959 of NUP100 locus inBamHI-SacIThis study
 pSW546Fragment encoding amino acids 726–1113 of NUP116 locus inBamHI-SacIThis study
 pSW547Fragment encoding amino acids 726–919 of NUP116 locus inBamHI-SacIThis study
 pSW548Fragment encoding amino acids 914–1113 of NUP116 locus inBamHI-SacIThis study
 pSW560Fragment encoding amino acids 914–1113 of NUP116 locus with 998–1105 replaced by G in BamHI-SacIThis study
pGBD backbone
 pSW1211Fragment encoding amino acids 998–1105 of NUP116 locus inBamHI-SalIThis study
 pSW1222Full-length NUP159 locus inEcoRIThis study
pBJ382 backbone
 pSW171Fragment encoding amino acids 726–1113 ofNUP116 locus inBamHI-SacI 28
  • a Vector backbone references (in parentheses): pGAD and pGBD (41); pGBT (5); pBJ382 (gift from C. Hug, Washington University, St. Louis, MO); pCITE (Novagen).