Table 2.

Targeted integration frequencies

GenotypeFrequency (%)afor targeting construct
XRCC2-puroOv-purob Ov-neob KU70-hyg
Wild type12/20 (60)24/37 (65)18/36 (50)18/37 (49)
rad51c 2/48 (4.2)5/46 (11)NDND
rad51c + hRad51C 2/24 (8.3)
rad51d 0/380/22NDND
rad51d + mRad51D 23/27 (85)
xrcc2 NDND0/340/43
xrcc2 + hXRCC2 3/40 (7.5)
xrcc3 0/420/36NDND
xrcc3 + hXRCC3 11/22 (50)
  • a Data are numbers of targeted clones at each locus per number of drug resistant clones analyzed. The percentage of targeted integration events is given in parentheses. The frequencies of transformation are essentially identical in wild-type and all mutant lines (∼40 clones per 107 transfected cells). ND, not determined.

  • b Two targeting constructs of the ovalbumin locus contain either puromycin or neomycin resistance genes.