Table 5.

Activities of eIF4GI mutant polypeptides

eIF4GI mutantBinary-complex formationBinding to eIF4ATernary-complex formation48S complex formationBinding to eIF3
eIF4GI(613–1090)+++++++++
eIF4GI(643–1076)++++++
eIF4GI(697–1076)+++++++++++++++
eIF4GI(722–1076)+++++++++
eIF4GI(734–1076)+++
eIF4GI(746–1076)++++
eIF4GI(772–1076)
eIF4GI(697–969)+++++
eIF4GI(697–949)+++++
eIF4GI(697–941)
eIF4GI mut1+++−/+
eIF4GI mut4+++−/+++
eIF4GI RRM1−/++++±−/+
eIF4GI RRM2++++++++++
eIF4GI mut(I749T, R754I)±+++±
eIF4GI mut796-Ins8±−/+++
eIF4GI mut(R855A)++++++++++++
eIF4GI mut889-Ins6++++++++++++
eIF4GI mut(R915I)±+++++