Table 4.

Binding affinities of EMCV nt 680 to 786 RNA to eIF4GI mutants in the absence and in the presence of eIF4A

eIF4GI mutantKd (nM)
−eIF4A+eIF4A
eIF4GI(643–1076)1705
eIF4GI(697–1076)4006
eIF4GI(722–1076)60080
eIF4GI(734–1076)700650
eIF4GI(746–1076)1,4001,300
eIF4GI(772–1076)>6,000>6,000
eIF4GI(697–969)2,30040
eIF4GI(697–949)2,800400
eIF4GI(697–941)3,0003,000
eIF4GI(697–869)>8,000>8,000
eIF4GI mut1200200
eIF4GI mut412030
eIF4GI RRM11,800400
eIF4GI RRM248010
eIF4GI mut(I749T, R754I)1,000200
eIF4GI mut796-Ins81,200400
eIF4GI mut(R855A)5008
eIF4GI mut889-Ins68005
eIF4GI mut(R915I)1,700300
p97(NAT1) 62–330>4,000>4,000