Table 3.

Binding affinities of β-globin RNA to eIF4GI mutants

eIF4GI mutant Kd (nM)
eIF4GI(643–1076)100
eIF4GI(697–1076)500
eIF4GI(722–1076)300
eIF4GI(734–1076)550
eIF4GI(746–1076)500
eIF4GI(772–1076)500
eIF4GI(800–1076)600
eIF4GI(697–969)1,000
eIF4GI(697–949)2,800
eIF4GI(697–941)2,600
eIF4GI(697–869)6,500
eIF4GI(643–696)200
eIF4GI mut1400
eIF4GI mut4300
eIF4GI RRM1800
eIF4GI RRM2550
eIF4GI mut(I749T, R754I)1,500
eIF4GI mut796-Ins8700
eIF4GI mut(R855A)800
eIF4GI mut889-Ins61,100
eIF4GI mut(R915I)1,000
p97(NAT1) 62–330>4,000