Table 1.

Yeast strains constructed for this study

StrainRelevant genotypeaPlasmid
YCB461 MAT a trp1Δ pRP485
YBL4145 MAT a spb10-1 pab1Δ::HIS3
YCB557 MATα spb10-1
YCB451 MATα spb10-1 pFL44-A1
YCB452 MATα spb10-1 pFL44-A1K
YCB571 MAT a /α pat1Δ::TRP1/PAT1
YCB499 MAT a pat1Δ::TRP1
YCB467 MAT a pat1Δ::TRP1 pab1Δ::HIS3 pAS77
YCB573 MAT a /α pat1Δ::TRP1/spb10-1
YCB572 MAT a /α pat1Δ::TRP1/pat1Δ::TRP1
YCB482 MATα pat1Δ::TRP1 pRP 485
YCB574 MAT a /α mrt1-3 pat1Δ::TRP1
YCB575 MAT a /α mrt3-1 pat1Δ::TRP1
YCB562 MAT a dcp1Δ::URA3 pat1Δ::TRP1
YCB563 MATα xrn1Δ::URA3 pat1Δ::TRP1
YBL4452 MAT a spb8Δ::LEU2
YCB568 MAT a spb8Δ::LEU2 pat1Δ::TRP1
YCB536 MATα PAT1-protA::TRP1,pep4::URA3
  • a All strains are ade2-1 his3-11 leu2-3,112 trp1-1 or trp1Δ ura3-1 can1-100, unless otherwise stated.