Table 2.

Phenotypic summary of F2 generation mice

Mouse IDaAge (mo)aSexaEnlarged spleenbKidney infiltratescLung infiltratescIgG depositsdProlapsed rectumbUrine analysise (protein/blood)
Emk +/+
 171 11M N+ +NNDf
 23211 M N N ND
 250 7M N + N −/−
 252 12 MN ++ N −/−
 121 7 F NND N ND
 166 12 F N ++ ++ N −/−
 159 8 F N + N ND
 242 8 F N N ND
 179 7 F N + ++ N−/−
 194 8 F N + Ntrace/−
 253 11 F N ND NND
 254 11 F N + N ND
Emk +/−
 170 11 M Y ND + Y ND
 172 11 M Y ++ ++ N ND
 199 12 M N + + N trace/−
 251 12 M N + +N −/−
 306 12 F N ++ + +++ N−/−
 173 12 F N ++ + ++ Ntrace/−
 175 12 F N + + ++ N−/−
 158 10 Y ++ ++++ NND
Emk −/−
 164 7 M Y ++++ Y −/250
 250 12 M N +++ N trace/−
 293 7 M Y +++ + Y trace/−
 202 12 M N ++++ ++ N 30/50
 212 12 F N +++++ + N trace/−
 195 12 F N +++ + N trace/−
 307 12 F N +++++ ++++ N −/50
 119 7 F Y ++++++++ ND Y ND
 127 8 F Y ++++++++ ++ Y −/50
 144 8 F Y ++++++ ++++ Y −/250
 1497 F Y +++ +++ +++++ N 500/250
 1847 F Y +++ Y −/250
 2358 F Y + + + Y −/250
  • a Mice are specified by an identification (ID) number, and their sex and age (male [M] or female [F]) are indicated.

  • b The presence (Y) or absence (N) of prolapsed rectum and enlarged spleen is indicated. Spleen enlargement was evaluated by weight and/or total splenocyte counts (see the text).

  • c Hematoxylin and eosin staining was used to detect lymphocytic infiltrates in the kidneys and lungs; − no infiltrate; +, minor infiltrate; ++++, maximal infiltrate.

  • d IgG deposition on the kidneys was determined by indirect immunofluorescence using anti-mouse IgG antibody conjugated to Cy3. Frozen kidney sections were evaluated for intensity and frequency of immunofluorescent glomeruli, and those data were used to determine a score (see Fig. 4C and D for examples of no deposition and maximal deposition, respectively); − no IgG deposition; +, minor deposition; +++++, maximal deposition.

  • e Urine was analyzed for the presence of protein (values on the left) and blood (values on the right). Protein levels range from undetectable (−) to 30–500 mg/dl, and hemoglobin levels varied from undetectable (−) to 50–250 erythrocytes/ml.

  • f Samples that were not evaluated are indicated by ND.