Table 1.

Quantitation of T- and B-cell populations in immune tissues of Emk +/+ andEmk −/− micea

Tissue Genotype Total cell no. ± SD Total no. of CD4+ cells ± SD (% of total)Total no. of CD8+ cells ± SD (% of total)Total no. of B220+ cells ± SD (% of total)
7–8-wk-old mice
 Spleen Emk +/+ (17.60 ± 6.20) × 107  (n = 9)(2.86 ± 1.25) (16.2) × 107  (n = 8)(1.58 ± 0.57) (8.9) × 107  (n = 8)(9.21 ± 3.89) (52.3) × 107  (n = 9)
Emk −/− (12.10 ± 5.30) × 107  (n = 10)(1.85 ± 0.82) (15.3) × 107  (n = 8)(1.21 ± 0.59) (10.0) × 107  (n = 8)(5.25 ± 2.60) (43.3) × 107  (n = 10)
 Lymph node Emk +/+ (3.50 ± 2.00) × 106  (n = 6)(1.77 ± 1.13) (50.5) × 106  (n = 6)(0.73 ± 0.53) (20.8) × 106  (n = 6)(0.61 ± 0.50) (17.4) × 106  (n = 6)
Emk −/− (6.00 ± 2.58) × 106  (n = 7)1 (2.29 ± 1.26) (38.2) × 106  (n = 7)(1.32 ± 0.60) (22.0) × 106  (n = 7)(1.53 ± 0.85) (25.5) × 106  (n = 7)2
 Thymusd Emk +/+ (14.70 ± 7.9) × 107  (n = 7)(1.33 ± 0.96) (10.0) × 107  (n = 7)(0.45 ± 0.16) (3.4) × 107  (n = 7)
Emk −/− (13.90 ± 6.4) × 107  (n = 10)(1.27 ± 0.80) (9.1) × 107  (n = 8)(0.41 ± 0.23) (3.0) × 107  (n = 8)
7–12-m-old mice
 Spleen Emk +/+ (14.82 ± 4.98) × 107  (n = 11)(2.85 ± 1.22) × 107 (19.2)  (n = 10)(1.24 ± 0.57) (8.4) × 107  (n = 8)(5.98 ± 3.55) (40.4) × 107  (n = 11)
Emk −/− b (33.13 ± 11.62) × 107  (n = 8)3 (4.63 ± 3.56) × 107 (14.0)  (n = 8)(1.81 ± 0.94) × 107 (5.5)  (n = 7)(5.09 ± 4.06) (15.4) × 107  (n = 8)
Emk −/− (10.15 ± 4.36) × 107  (n = 8)(1.98 ± 0.92) × 107 (19.5)  (n = 6)(0.92 ± 0.38) × 107 (9.1)  (n = 5)(4.34 ± 2.92) (42.8) × 107  (n = 8)
 Lymph node Emk +/+ (3.66 ± 4.54) × 106  (n = 9)(1.67 ± 2.31) × 106 (45.6)  (n = 6)NDc (0.94 ± 0.81) (25.6) × 106  (n = 7)
Emk −/− c (9.90 ± 6.35) × 106  (n = 6)2 (3.09 ± 1.92) × 106 (31.2)  (n = 4)ND (5.74 ± 4.98) (58.0) × 106  (n = 4)4
Emk −/− (7.07 ± 3.9) × 106  (n = 6)1 (2.06 ± 1.09) × 106 (29.1)  (n = 6)ND (2.57 ± 1.95) (36.4) × 106  (n = 6)2
  • a Quantitation of T- and B-cell populations in immune tissues of Emk +/+ andEmk −/− mice. Cell suspensions prepared from spleen, thymus, and superficial inguipal lymph nodes were analyzed by FACS using antibodies specific for CD4, CD8, and B220. A minimum of four mice per group was analyzed (the number is shown as n). Numbers indicate significant differences betweenEmk +/+ and Emk −/− mice after analysis using a Student t test; 1, P < 0.10 ; 2, P < 0.05 ; 3, P < 0.005 ; 4, P < 0.025 .

  • b Emk −/− with splenomegaly/colorectal prolapse.

  • c ND, not done.

  • d For CD4+/CD8+ cells, values are as follows: Emk +/+, (11.9 ± 7.00) (80.9) × 107 (n = 7);Emk −/−, (11.0 ± 5.20) (79.1) × 107 (n = 8).