Table 1.

Steady-state kinetic parameters for nucleotide incorporation opposite the 3′T of the (6-4) TT photoproduct by hPolη

TemplateTemplate nucleotideIncoming residueVmax (nM/min)Km (μM)Vmax/Km finc
NondamagedTG1.4 ± 0.069.4 ± 2.10.159.5 × 10−3
A1.1 ± 0.050.07 ± 0.0215.71
T1.1 ± 0.0926 ± 70.0422.7 × 10−3
C1.3 ± 0.0753 ± 80.0251.6 × 10−3
(6-4) TT3′ T of (6-4) TT photoproductG0.9 ± 0.023.2 ± 0.50.287.8
A0.9 ± 0.00525 ± 0.60.0361
T1.0 ± 0.0584 ± 120.0120.33
C1.3 ± 0.289 ± 370.0150.42