Table 2.

Steady-state kinetic parameters for nucleotide incorporation opposite the 3′ T of the (6-4) TT photoproduct by yPolη

TemplateTemplate nucleotideIncoming residueVmax (nM/min)Km (μM)Vmax/Km finc
NondamagedTG1.5 ± 0.0670 ± 70.0217.2 × 10−3
A1.4 ± 0.10.48 ± 0.22.91
(6-4) TT3′ T of (6-4) T-T photoproductG1.4 ± 0.0347 ± 40.037.5
A0.8 ± 0.08180 ± 420.0041