Table 1.

Yeast strains

StrainGenotypeSource or reference
W303-1a MAT a leu2-3,112 ura3-52 his3-11,15 ade2-1 trp1-1 can1-100
W303-1b MATα leu2-3,112 ura3-52 his3-11,15 ade2-1 trp1-1 can1-100
NQ6811 MAT a leu2-3,112 ura3-52 trp1Δ1 his4-912δlys2-128δ CDC68-200N POB3-200N, expressing HA-Cdc68 and HA-Pob3 proteins5
NB6241W303-1b withNHP6A-V5-His6 replacingNHP6A p303-NΔXN transformant
RJY6249 MAT a leu2-3,112 ura3-1 his3-11,15 ade2-1 trp1-1 can1-100 adl1Δnhp6a::URA3Δnhp6b::LEU2 36
NB6040RJY6249 withhis3::HIS3 pRS303 transformant
BM404 MAT a ura3-52 his4-912δlys2-128δ suc2ΔUAS F. Winston
DE4B-20a MATα cdc68922 leu2-3,112 ura3-52 trp1-1 his4-912δlys2-128δDE4B (13) segregant
DE4B-20b MAT a leu2-3,112 ura3-52 trp1-1 his4-912δ lys2-128δDE4B (13) segregant
NB4B-1c MATα cdc68922 leu2-3,112 ura3 trp1-1 his4-912δ Δnhp6a::URA3Δnhp6b::LEU2 NB6040 × DE4B-20a segregant
NB4B-3b MATαcdc68922 leu2-3,112 ura3 trp1-1 lys2-128δ Δnhp6a::URA3Δnhp6b::LEU2 NB6040 × DE4B-20a segregant
AW11-9a MATα leu2-3,112 ura3-52 trp1-1 his4-912δ lys2-128δsuc2ΔUAS BM404 × DE4B-20a segregant
NB39-11a MATα leu2-3,112 ura3 trp1-1 ade2-1 his4-912δ lys2-128δ Δnhp6a::URA3Δnhp6b::LEU2 suc2ΔUAS NB6040 × AW11-9a segregant
NB39-18a MAT a leu2-3,112 ura3 trp1-1 ade2-1 his4-912δ lys2-128δ Δnhp6a::URA3Δnhp6b::LEU2 NB6040 × AW11-9a segregant
NB39-24b MAT a leu2-3,112 ura3 trp1-1 his4-912δ lys2-128δ Δnhp6a::URA3Δnhp6b::LEU2Ade+ NB6040 × AW11-9a segregant
FY300 MAT a leu2Δ1 ura3-52 his4-912δ lys2-128δ spt5-194Ade+ 49
FY1671 MATαleu2Δ1 ura3-52 his4-912δlys2-128δ Δppr2::hisG 19
NB2Δp-28b MATα leu2 ura3-52 trp1-1 his4-912δ lys2-128δ Δppr2::hisGΔnhp6a::URA3 NHP6BAde+ (FY1671 × NB39-24a) × NB39-24a segregant
NB40NB39-18a × AW11-9a
NB40-1aF MAT a leu2-3,112 ura3 trp1-1 his4-912δ lys2-128δ Δnhp6a::URA3Δnhp6b::LEU2 withPOB3-NHP6A-V5-His6 replacingPOB3 NB40 segregant transformed with pPNT
FY711 MATα leu2-3,112 ura3-52 his4-912δ lys2-128δsnf5-5::URA3 4
NB6244W303-1a with POB3-NHP6A-V5-His6 replacingPOB3 pPNT transformant
NB6249RJY6249 withPOB3-NHP6A-V5-His6 replacingPOB3 pPNT transformant
NB6251NB6249 withtrp1::HIS3 pTH4 (11) transformant