Table 1.

Q-FISH and cytogenetic analysis of wild-type and DNA-PKcs−/− MEFs

LitterGenotypeChr.aarmTelomere length (kb) (SD, SE)No. of chr. aberrationsNo. of metaphases
Telomere fusionsbChr. breaks or fragmentscTelomere associationsd
A1−/−p28.24 (9.78, 0.2)647100
q33.66 (11.38, 0.23
Avg30.95 (10.58, 0.219)
A2+/−p29.5 (9.41, 0.19)6214100
q34.92 (10.6, 0.021)
Avg32.21 (10.01, 0.2)
B6−/−p31.65 (10.73, 0.219)7221100
q36.5 (10.24, 0.209)
Avg34.08 (10.48, 0.21)
B5WTe p32.61 (11.17, 0.22)2213100
q37.7 (10.03, 0.2)
Avg35.15 (10.6, 0.21)
C2−/−p34.56 (9.49, 0.19)6419100
q40.27 (11.13, 0.22)
Avg37.42 (10.31, 0.21)
C5WTp30.81 (8.61, 0.17)141080
q38.97 (9.94, 0.2)
Avg34.89 (9.28, 0.19)
C3WTp31.58 (10.54, 0.21)2310100
q38.45 (11.8, 0.24)
Avg35.01 (11.17, 0.22)
C9−/−p27.78 (8.22, 0.16)601180
q32.92 (9.96, 0.2)
Avg30.35 (9.09, 0.18)
  • a Chr., chromosome.

  • b Average numbers per metaphase: wild type, 0.017; DNA-PKcs−/−, 0.065.

  • c Average numbers per metaphase: wild type, 0.032; DNA-PKcs−/−, 0.026.

  • d Average numbers per metaphase: wild type, 0.012; DNA-PKcs−/−, 0.015.

  • e WT, wild type.