Table 2.

Summary of CD mutational dataa

CD proteinEquivalent position in swi6Consensus residueMutationActivitySource or reference
HP1E78–E80EEE-AAAYes 50
swi6E74–E80EEEEEDE-AAAAAAAResidualThis study
swi6E74–E80EEEEEDE-RRRRRRRResidualThis study
HP1Y81Y%Y-FNo 50
HP1V83V V-MNo 50
swi6E84E-FYesThis study
PcV86 I-F (Pc106 )No 41
HP1H89R90+RR-QQYes 50
swi6M91A92 ∗∗ MA-VVYesThis study
swi6M91A92 ∗∗ MA-QGNoThis study
HP1K94+K-QYes 50
swi6Y100Y107Y%YY-CCNoThis study
swi6L101L102L%LL-NNNoThis study
swi6K103W104+WKW-VVNoThis study
swi6K103+K-QYesThis study
clr4W104WW-GPartial 29
clr4W115WW-GPartial 29
swi6W115WW-GResidual or partialThis study
swi6N113–W115N(T/S)WDeletionResidualThis study
PcI128–D129 0Deletion (PcXL5 )No 41
  • a +, Conserved basic residues; −, conserved acidic residues; ∗, conserved hydrophobicity; %, semiconserved hydrophobicity; 0, no clear conservation. For the activities of mutants used in this study, “yes” corresponds to >50% wt activity, “partial” corresponds to 20 to 50% wt activity, “residual” corresponds to 10 to 20% wt activity, and “no” corresponds to <10% wt activity in asci formation and MCLR assays (see Table 1).