Table 2.

Quantification of the ScKar2p (wild-type and mutant) fractions bound to GST-ScSls1p or GST-ScSls1.5p in pulldown assays expressed as a percentage of total inputa

Fraction% Total input
Kar2pG246DG247DG274DT59GT249G
ATPADPATPADPATPADPATPADPATPADPATPADP
GST-Sls1p267490100909410010029784152
GST-Sls1.5p08655537343526302900
  • a GST binding assays were as in Fig. 6. Proteins bound to the glutathione pellet or remaining in the supernatant were quantified by scanning densitometry with NIH Image 1.61 software (values are an average of three independent experiments).