Table 1.

Telomere FISH analyses of GM847 and GM847/hTERT cells

Cell typeMetaphases with at least one undetectable telomere (%)aNo. of undetectable telomeres (%)b
GM847 untransfected 50/50 (100)41/704 (6)
GM847/hTERT-3 (PD 42) 12/50 (24)
GM847/hTERT-3 (PD 106) 9/50 (18) 8/690 (1)
GM847/hTERT-3 (PD 138) 13/50 (26)
GM847/hTERT-6 (PD 46) 13/55 (23)
GM847/hTERT-6 (PD 82) 15/50 (30)1/894 (0.1)
GM847/hTERT-6 (PD 150) 13/52 (25)
GM847/hTERT-3 subclone 9 1/50 (2) 2/1,232 (0.2)
GM847/hTERT-6 subclone 11 0/50 (0) 2/1,210 (0.2)
  • a Metaphases were scored as containing at least one chromosome end without a detectable telomere FISH signal.

  • b Number of undetectable telomeres relative to the total number of chromosome ends analyzed by scoring every chromosome end in five additional (i.e., different) metaphases.