Table 1.

Cell-type-specific induction of antiapoptotic genes by TNF

Genem-RNA intensity and expression ratio for indicated cell typea
SV80Kym-1MCF7HeLa293JurkatKB
UntreatedTNFUntreatedTNFUntreatedTNFUntreatedTNFUntreatedTNFUntreatedTNFUntreatedTNF
cFLIP7011,716 (3.3)102183 (3.3)42146 (3.9)75174 (2.9)5539 (0.7)7346 (0.85)88172 (2.1)
L321,5231,142 (0.75)6,2193,435 (0.55)3,0082,707 (0.90)2,1951,756 (0.8)2,1392,601 (1.22)1,8951,422 (0.75)1,6071,509 (0.94)
TRAF11432,763 (20)ND13 (induced)NDND (NI)ND10 (induced)NDND (NI)NDND (NI)ND40 (induced)
cIAP233345 (11)311 (11)429 (8.4)224 (12)NDND (NI)830 (5)11127 (14)
cIAP182238 (3)3550 (4)1613 (0.9)721 (3)2136 (1.5)1310 (1.1)2498 (5)
L321,5531,523 (0.98)575188 (0.33)3,4933,004 (0.86)2,1732,135 (0.98)3,3463,955 (1.18)1,128832 (0.73)2,2761,821 (0.8)
  • a mRNA intensities are already corrected for background levels. To calculate relative expression levels (parenthetical numbers in rows 1 and 3 to 5), the mRNA intensities of TNF-treated cells were divided by the mRNA intensities of untreated cells. To take into account differences in sample loadings, this ratio was finally corrected according to the ratio parenthetical numbers in rows 2 and 6 of the expression levels of the housekeeping L32 gene in treated and untreated groups. For example, TNF-induced cFLIP expression in SV80 cells was calculated as follows: 1,716/701 × 1,523/1,142 = 3.3. FLIP and TRAF1, cIAP1, and cIAP2 were analyzed with different template kits and corrected for the corresponding internal L32 control. ND, not determined. NI, not induced.