Table 3.

Percentage of cells showing nuclear or cytoplasmic staining of cyclin D1a

Cell typeTET% of cells showing staining of:
CytoplasmNucleus
V3/D1+3 ± 397 ± 4
V3/D13 ± 293 ± 4
S24/D1+4 ± 294 ± 4
S24/D198 ± 424 ± 5
S24/D1 + PMAc +NAd 95 ± 4
S24/D1 + PMANA78 ± 5
293T+D1-pBABEhygro(1:2.5)b 22 ± 686 ± 4
293T+D1-SSeCKS (1:2.5)78 ± 427 ± 4
293T+D1-pBABEhygro (1:10)35 ± 480 ± 2
293T+D1-SSeCKS (1:10)73 ± 618 ± 4
  • a Cells grown on 22-mm2coverslips under various TET conditions (+, with; −, without) for 2 days were fixed and stained for cyclin D1 using PAb, as described in Materials and Methods. Independent fields of cells were counted (total of 250 to 300 cells for each analysis).

  • b 293T cells were transfected transiently with Lipofectamine (GIBCO-BRL) containing pEGFP-1, pRcCyclin D1 (gift of A. Dutta) plus pBABEhyg, or pBABEhyg/SSeCKS at either a 1:2.5 or 1:10 ratio, fixed after 40 h, and then stained for cyclin D as described above.

  • c Cells grown with PMA (200 nM final concentration) for 30 min.

  • d NA, not applicable because PMA treatment decreased the cytoplasmic, but not nuclear area.