Table 1.

C-terminal signal sequences of unstable Ura3p fusion proteins stabilized in a ubc6 ubc7 background

Clone Deduced C-terminal sequencea
CL1ACKNWFSSLSHFVIHL
CL2SLISLPLPTRVK FSSLLLIRIMKIITMTFPKKLRS
CL6FYYPIWFARVLLV HYQ
CL9SNPFSSLFGASLLIDSVSLKSNWDTSSSSCLISFFSSVMFSSTTRRS
CL10CRQRFSCHLTASYPQSTVTPFLAFLRRDFFFL RHNSSAD
CL11GAPHVVLFDFELRITNPLSHIQSVSLQITLIFCSLPSLILSKFLQV
CL12NTPLFSKSFSTTCGVAKK TLLLAQISSLFFLLLSSNIAV
CL15PTVKNSPK IFCLSSSSPYLAFNLEYLSLRIFSTLSKCSNTLLTSLS
CL16SNQLKR LWVLWLLEV RSFDRTLRRPWIHLPS
SL17SISFVIRSHASIRMGASNDFFHKLYFTKCLTSVILSKFLIHLLL RSTPRV
  • a Hydrophobic regions in a Kyte-Doolittle hydropathy plot (window size = 7) are underlined, and positively charged residues are boldface.