Table 5.

Percentage of cells showing nuclear staining of cyclin D1 following treatment with penetratin-linked peptidesa

Cell typePeptide addedTET% of cells showing nuclear staining
S24/D1--+95 ± 3
S24/D1--28 ± 4
S24/D1Penetratin-wt CY82 ± 5
S24/D1Penetratin-K→S30 ± 5
S24/D1Penetratin-phospho-Ser507/515 39 ± 4
S24/D1wt CY95 ± 4
S24/D1K→S95 ± 5
S24/D1Phospho-Ser507/515 91 ± 5
  • a Cells grown on 22-mm2coverslips with (+) or without (−) TET for 3 days and then with or without peptide (100 μg/ml) for 4 h were fixed and stained for cyclin D1 using PAb, as described in Materials and Methods. Three independent fields of cells were counted (total of 250 to 300 cells for each analysis).