Table 2.

Establishment of rescued oriP plasmid populations in 293 cells

PlasmidAvg no. of drug-resistant coloniesaNo. of transfected cells plated (103)bCloning efficiency (%)bEstablishment efficiency (%)b
p22057.53.35.6
49.02.7315.9
60.53.55.6
rescued p22036.02.4314.8
58.02.76.9
67.53.46.4
46.02.85.2
39.03.04.2
51.03.15.3
p220ΔDS3.0c 3.2310.3
3.0c 3.30.3
1.0c 3.40.09
pHEBo1.03.2310.1
1.03.30.1
3.02.90.3
  • a Numbers reflect the average of 2 dilutions of cells plated.

  • b Transfection, cloning, and establishment efficiencies are as defined in footnotes b, c, andd of Table 1.

  • c Number reflects a single dilution of cells plated.