Table 1.

CaaX farnesylation sequence affects binding properties of Pex19pa

BD-hybridOptical density
AD-Pex19pAD-Pex19pΔCaaX
Pex3p163162
Pex16p22.215.1
Pex12p0.5400.002
Pex13p0.4310.004
Pex11pβ0.2680.030
Pex10p0.1990.038
  • a SFY526 yeast cells, transformed with plasmids encoding one of the indicated Ga14p DNA-BD fusion proteins (BD-hybrid) and the Ga14p AD fused to either Pex19p or Pex19pΔCaaX, were selected for leucine and tryptophan prototrophy. Double transformants were assayed for β-galactosidase usingo-nitrophenyl-β-d-galactopyranoside as substrate. The optical densities were measured at 421 nm and normalized for culture densities (optical density at 600 nm = 10) and time (24 h). The values given are the means of three measurements performed on independent single colonies.