Type of expression | Log_{10} ratio (fold) | No. of responding genes | |||||
---|---|---|---|---|---|---|---|

WT ± 3AT^{a} | GCN4/gcn4Δ ratio^{b} | GCN4/GCN4^{c}ratio^{c} | gcn4Δ ± 3AT^{d} | ||||

Set A | Set B | Set C | |||||

Induced | ≥10 | 23 | 44 | 99 | 63 | 7 | 18 |

≥4 | 164 | 177 | 322 | 210 | 54 | 80 | |

≥2 | 735 (18%)^{e}
| 771 (16%) | 949 (24%) | 612 (20%) | 158 (26%) | 568 (18%) | |

<2 | 2,481 | 2,991 | 2,991 | 1,836 | 452 | 2,186 | |

Repressed | ≥10 | 9 | 37 | 52 | 14 | 3 | 5 |

≥4 | 225 | 336 | 347 | 101 | 8 | 72 | |

≥2 | 886 (22%) | 955 (20%) | 1,092 (28%) | 636 (21%) | 65 (11%) | 496 (15%) | |

Total ORFs considered^{f}: | 4,102 | 4,717 | 3,940 | 3,087 | 610 | 3,250 |

↵a Expression in WT strain treated with 100 mM 3AT relative to expression in untreated strain. Set A, average expression ratio from 11 independent pairs of hybridizations; set B; average expression ratio from 8 independent pairs of hybridizations; set C; average expression ratio from 2 independent pairs of hybridizations. Sets A and B and

*GCN4/GCN4*ink-jet data sets were derived from cDNA arrays, and sets C and^{c}*gcn4*Δ ± 3AT data sets were derived from the use of FlexJet oligonucleotide arrays. Data sets A and B were derived from two separate RNA preparations from strain R491. Data set C was obtained from strain KNY 164. All strains are in an S288c background.↵b Expression ratio of a WT

*GCN4*strain (KNY164) to an isogenic*gcn4*Δ strain (KNY124), both treated with 100 mM 3AT.↵c Expression ratio of strain R4760 overexpressing

*GCN4*constitutively (*GCN4*) to strain R6257 bearing vector alone (^{c}*GCN4*).↵d Expression ratio of

*gcn4*Δ strain (KNY124) treated with 100 mM 3AT to the untreated strain.↵e Values in parentheses are percentages of total ORFs considered.

↵f Total number of ORFs that are above the

*P*value cutoff ( P = 0.05 ).