TABLE 1

Characteristics of the available LepA structures in complex with 70S ribosomes

Structure informationPRE complexesPOST complexes
LepA-GDPNP-PRELepA-GDPNP-PRE (major group)LepA-GDPCP-PRELepA-GDP-POSTLepA-GDPNP-POST (minor group)LepA-GDPCP-POST (major group)LepA-GDPCP-POST (minor group)
Reference25231527233535
MethodCryo-EMCryo-EMX-ray crystallographyX-ray crystallographyCryo-EMCryo-EMCryo-EM
Resolution (Å)10.93.22.62.93.73.85.7
OrganismEscherichia coliEscherichia coliThermus thermophilusThermus thermophilusEscherichia coliThermus thermophilusThermus thermophilus
mRNA (nt)MF-mRNAa (46), AUG-UUCMF-mRNA (81), SDb (GGAGG) AUG-UUCMF-mRNA (24), SD (GGAGG) AUG-UUCmRNA (18), SD (GGAGG) UUCMF-mRNA (81), SD (GGAGG) AUG-UUCMF-mRNA (25), SD (GGAGG) AUG-UUCMF-mRNA (25), SD (GGAGG) AUG-UUC
A-tRNA (state)Ac[14C]Phe-tRNAPhe (A/L)Ac[14C]Phe-tRNAPhe (A/4)Phe-tRNAPhe (A/L)NoneNoneNoneNone
P-tRNA[32P]deacyl-tRNAfMetDeacyl-tRNAfMetfMet-tRNAfMetPhe-tRNAPheAc[14C]Phe-tRNAPhetRNAfMettRNAfMet
E-tRNANoneNoneNonetRNAPhe (modeled)Deacyl-tRNAfMettRNAPhetRNAPhe
30S rotation (°)UnrotatedUnrotatedUnrotatedClockwise (5)Clockwise (4)UnrotatedCounterclockwise (5.6)
PDB ID3DEG3JCE5J8B4W2E3JCD51MQ51MR
PrepnLepA and GDPNP incubated with POST state 70SLepA and GDPNP incubated with POST state 70SLepA and GDPCP incubated with PRE state 70SLepA and GDPCP incubated with 70S and P-site Phe-tRNAPhePRE state 70S incubated with EF-G to yield POST state; LepA and GDPNP incubated with POST state 70SLepA and GDPCP incubated with PRE state 70SLepA and GDPCP incubated with PRE state 70S
CommentsMissing last 55 aa of CTDNoneEngineered L9 fusion; highest-resolution structure so farEngineered L9 fusionOnly modeled 26 residues of CTDEngineered L9 fusionEngineered L9 fusion
  • a MF-mRNA, mRNa containing the codons for methionine (M) and phenylalanine (F).

  • b SD, Shine-Dalgarno element. The sequences of the SD (in parentheses) and the codons are indicated.