Table 2.

Plasmids used in this study

PlasmidDescriptionReference or source
pDLB377 LEU2 GAL1p-CDC42 See text
pDLB642pCR2.1CDC42p-TDH3t See text
pDLB643pCR2.1CDC42p-CDC42-TDH3t See text
pDLB644CEN URA3 CDC42p-TDH3t See text
pDLB7222μm URA3 CLA4 Erfei Bi
pDLB1034pUNI-10CDC42-Q61L See text
pDLB1035pUNI-10CDC42-D57Y See text
pDLB1160pUNI-10CDC42-V36A,Q61L See text
pDLB1234pHB1-MYC3 CDC42-D57Y See text
pDLB1235pHB1-MYC3 CDC42-Q61L See text
pDLB1240pHB1-MYC3 CDC42-V36A,Q61L See text
pDLB1242pHB1-MYC3CDC42-Y40C,Q61L See text
pDLB1277pUNI-10 CDC42-Y40C,Q61L See text
pG11-4CEN URA3 GAL1p-STE11-4 See text
pGEX-Ste20CRIB GST-STE20-CRIB See text
pGS11ΔN-TCEN TRP1 GAL1p-GST-STE11ΔN See text
pGFP-GS5ΔN-CTMCEN TRP1 GAL1p-GFP-STE5ΔN-CTM 40
pGFP-GS5ΔN-Sec22CEN TRP1 GAL1p-GFP-STE5ΔN-Sec22 40
pL19CENURA3 GAL1p-STE4 50
pL-GS5ΔN-CTMCEN LEU2 GAL1p-STE5ΔN-CTM 40
pMOSB16pCR2.1cdc42-V36A See text
pMOSB29 LEU2 GAL1p-CDC42-D57Y See text
pMOSB36CEN URA3 cdc42-md1 See text
pMOSB37CEN URA3 cdc42-md2 See text
pMOSB38CEN URA3 cdc42-md12 See text
pMOSB42CEN TRP1 cdc42-md1 See text
pMOSB43CEN TRP1 cdc42-md2 See text
pMOSB44CEN TRP1 cdc42-md12 See text
pMOSB45CEN TRP1 CDC42 See text
pMOSB47CEN TRP1 cdc42-V36A See text
pMOSB53pCR2.1cdc42-Y40C See text
pMOSB55CEN URA3 CDC42 See text
pMOSB134 URA3 ste20ΔCRIB See text
pMOSB175CEN TRP1 cdc42-Y40C See text
pMOSB176CEN URA3 cdc42-Y40C See text
pMOSB177CEN URA3 cdc42-V36A See text
pNC2522μm URA3 GAL1p-STE12 39
pPB3212μmURA3 BEM1 5
pPP828CENLEU2 GFP-STE20 See text
pRL116CEN URA3 GFP-STE20 23
pSB231CEN URA3 FUS1-lacZ 48
pVTU-Ste202μmURA3 ADH1p-STE20 21
p3058CENLEU2 FUS1p-lacZ C. Boone