Table 1.

S. pombe strains used in this study

Strain or mutationGenotypeSource
972 h P. Nurse
cdc20-M10 h cdc20-M10 leu1-32 ura4-D18 P. Nurse
cdc20-P7 h cdc20-P7 leu1-32 P. Nurse
Δcdc20/cdc20+ h/h+cdc20+/cdc20::ura4+ade6-M216/ade6-M210 leu1-32/leu1-32 ura4-D18/ura4-D18 P. Nurse
cdc20 ΔN-term h cdc20::ura4+ade6-704 leu1-32 ura4-D18 int pJK148-nmt41-3hacdc20C1 intpRep6X This study
cdc20N-term+C-term hcdc20::ura4+ ade6-704 leu1-32 ura4-D18 int pJK148-nmt41-3hacdc20C1 intpRep6X-cdc20N This study
cdc203hacdc20+ hcdc20::ura4+ ade6-704 leu1-32 ura4-D18 int pJK148-nmt41-3hacdc20+ This study
cdc20tsN-term+C-term hcdc20::ura4+ ade6-704 leu1-32 ura4-D18 int pJK148-nmt41-3hacdc20C1 intpRep6X-cdc20N(M10) This study
Δhus1 hhus1::leu2+ ade6-704 leu1-32 ura4-D18 T. Enoch
Δrad3 h rad3::ura4+ade6-704 leu1-32 ura4-D18 A. Carr
Δcds1 h cds1::ura4+ade6-704 leu1-32 ura4-D18 P. Nurse
Δchk1 h chk1::ura4+ade6-704 leu1-32 ura4-D18 P. Nurse
cdc20N-term+C-term Δchk1 h+ cdc20::ura4+chk1::ura4+ ade6-704 leu1-32 ura4-D18 int pJK148-nmt41-3hacdc20C1 intpRep6X-cdc20N This study
cdc20tsN-term+C-term Δchk1 h+ cdc20::ura4+chk1::ura4+ ade6-704 leu1-32 ura4-D18 int pJK148-nmt41-3hacdc20C1 intpRep6X-cdc20N-M10 This study