Table 1.

Kinetic constants determined by curve fitting (Fig. 2) using GST RAG proteinsa

DNA substrateCoding end sequenceK12 or K23(nM)kN (min−1)
KY28/295′-TAGCTAC-12RSS-3′600.6
KY36/375′-ACAGTAG-23RSS-3′670.5
  • a Coding end sequences in the second column represent the top strand sequence (Fig. 1).K12 and K23 are the Michaelis-Menten constants (Km) for the 12- and 23-substrate, respectively. kN is the catalytic constant (turnover number) for nicking. For MBP-RAGs,K12 = 59 nM andkN12 = 0.07 min−1;K23 = 45 nM andkN23 = 0.13 min−1.